Simplified assay method for 3-hydroxyanthranilic acid in human urine by fluorometry and its normal value.

نویسندگان

  • M Minami
  • K Mori
  • T Tabuchi
  • I Hara
چکیده

Estimations of human exposure levels to aromatic nitroand amino-compounds (ANA) are often made by Bratton-Marshall's diazo reaction. In Japan, the environmental conditions of the ANA industries have recently improved remarkably. The workers in ANA plants excrete somewhat higher levels of diazotizable substances in the urine than healthy controls and the contributory ratio of the exposed ANA substances to the total diazotizable ones in the urine appears to be of the order of 10 to 20%, most of the time. Under such circumstances, it is necessary to measure the contributory ratio of physiological ANA substances such as hydroxyanthranilic acid to the total diazotizable substances. A simplified assay method for hydroxyanthranilic acid in the urine was devised employing cation exchanging resins for the separation of the hydroxyanthranilic acid from other contaminating substances in the urine, and using fluorometry in alkaline solution for intensifying the fluorescence of hydroxyanthranilic acid. The reproducibility of the fluorimetric method was tested by an enzymatic assay method for hydroxyanthranilic acid determination in human urine, and the correlation coefficient was satisfactorily high (r••0.890, p•ƒ0.01). The normal excretory value of hydroxyanthranilic acid in the human urine ranges from 1.71 to 11.2

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عنوان ژورنال:
  • Industrial health

دوره 18 4  شماره 

صفحات  -

تاریخ انتشار 1980